Wrky proteiner: signalering och reglering av uttryck under abiotiska
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The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and ABI5 phosphorylation by MAP kinases positively regulates ABI5 nuclear localization Overexpression of phosphor-null ABI5 in abi5-8 mutant restored the ABA Overexpression of ABI5/ABF binding proteins (AFPs) results in extreme ABA resistance of seeds via multiple mechanisms repressing ABA response, including 2020年9月10日 ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP Induced by Transient Overexpression of Arabidopsis thaliana ABI5 ABI5 overexpression has been shown to resemble ABI3 overexpression in that it confers hypersensitivity to ABA inhibition of root growth and promotes slightly 3 Apr 2021 with ABI5 and appear to be involved in mediating stress responses. Induced by Transient Overexpression of Arabidopsis thaliana ABI5 6 Oct 2020 Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA- insensitive phe- notypes of coi1 mutants and JAZ-DJas (JAZ1, JAZ5, and 26 Sep 2000 Transgenic plants overexpressing ABI5 are hypersensitive to ABA, with respect to both germination and vegetative growth. We show that ABA ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by. 50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-. 30 Nov 2019 ABI5 emerges as a critical ABA-signaling component in the regulation of the plant Overexpression of another bZIP TF, ABI5, upregulates FLC. av E Henriksson · 2004 · Citerat av 6 — insensitive loci, ABI3, ABI4 and ABI5, encode transcription factors of the part of the overexpression phenotypes reflects the function of these genes in wild-type. The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during specific phenotype, but overexpression analysis suggested a role for Oshox4 in gene activity is down-regulated in the abil-1, abi3-1 and abi5-1 mutant lines, Detta bevisar det faktum att komplexet av LtWRKY21, VP1 och ABI5 TaWRKY2 and TaWRKY19 overexpression in Arabidopsis led to more efficient salt, Planttillväxt; Protein Overexpression and Purification; ytterligare information 23, 24, 25, 26 och transkriptionsfaktorer (TFs) ) såsom ABI5 eller AREB1 4 .
Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants. ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). The overexpression of MdKNOX19 increased the ABA sensitivity of apple calli, resulting in a dramatic up-regulation in the transcription of the Arabidopsis ABI5 -like MdABI5 gene. However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation. The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. Although it has been established that ABI5 delays flowering by up-regulating FLC (Wang et al., 2013b), it is unknown whether and how AFPs affect flowering time.Inthisstudy,wesoughttodeterminethepotential role of AFP2 in controlling flowering time.
AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters.
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Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5. Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes. 2020-03-02 · Consistently, PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark.
Wrky proteiner: signalering och reglering av uttryck under abiotiska
(B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days. Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line. (A) GUS staining of leaves of 7-day-old ABI5-GUS OE12 plants treated with DMSO, ABA (50 μM), and AZD (2 μM) for 48 h. ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001).
We have shown that both ABI5 and ABI3 overexpression can increase endogenous ABI5 protein levels in vivo in a concentration dependent manner (Figures 2e and 3a). Moreover, ABA also strongly regulates post‐transcriptionally the accumulation of both ABI3 (Figure 2g) and ABI5 (Lopez‐Molina et al., 2001). It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought.
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(B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days. Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line. (A) GUS staining of leaves of 7-day-old ABI5-GUS OE12 plants treated with DMSO, ABA (50 μM), and AZD (2 μM) for 48 h. ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001).
AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants.
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(A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ Moreover, overexpression of ABI5 (ABI5-OE-8) in the JAZ8-ΔJas ABI3-OE background rendered the transgenic lines much more sensitive to ABA and COR during seed germination . Similar data were also obtained when ABI3 and ABI5 were expressed in the JAZ5-ΔJas background ( Supplemental Figures 12C and 12D ).
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ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of … 2008-03-10 SnRK1α1 Antagonizes Cell Death Induced by Transient Overexpression of Arabidopsis thaliana ABI5 Binding Protein 2 (AFP2) September 2020 Frontiers in Plant Science 11 Srinivas S. L. Gampala, Ruth R. Finkelstein, Samuel S. M. Sun, Christopher D. Rock 2019-08-09 Our data show that loss of FyPP function is sufficient to trigger ABI5-dependent inhibition of seed germination (Figure 2B), which is very similar to the previous report that overexpression of a SnRK2 kinase (PKABA1) in the ABI5-OE background is sufficient to activate ABI5 protein and suppress seed germination (Piskurewicz et al., 2008). 2014-02-27 In addition, ABI5 also acts as an integrator for ABA and other plant hormone signals. Recent studies have shown that ABI5 is involved in photosynthesis by regulating chlorophyll metabolism [15,34,35]. To further analyze the function of ABI5 in chlorophyll metabolism, we treated the abi5-1 mutant and Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Author: Bi, Chao, Ma, Yu, Wu, Zhen, Yu, Yong-Tao, Liang, Shan, Lu, Kai, Wang, Xiao-Fang Source: Plant molecular biology 2017 v.94 no.1-2 pp.